Investigation of species composition of the fungi of the Fusarium genus and the resistance of the Chornobyl radio-mutants to fusarium head blight for the purposes of winter wheat

Aim. To investigate the field resistance of collection spelt-like samples Chornobyl radio-mutants (RM) winter wheat to Fusarium head blight and determine species composition fungi genus in Forest-Steppe Ukraine. Methods. The (in scores) ten RM-samples Fusar- ium was determined conditions by visual inspection plants using standard methods (Trybel et al, 2010). infection rate seeds, biological method seed germination rolls filtration paper used. DNA extracted reaction kit AGROSORB NK (Agrogen Novo, Ukraine), according manufacturer’s instructions. molecular identification conducted with commercial test systems “Fuzarioz zlakiv” (AgroDiagnostica, RU) instructions, real-time PCR. Results. results evaluating are presented. as 2016–2018 Ukraine, namely Kyiv, Cherkasy, Sumy, Vinnytsia, Chernivtsi, Poltava, Kharkiv, Ternopil, Khmelnytsky regions is study spe- cies fungi, 639 were taken from farms located territory nine zone. material demonstrate presence seven out eight under investigation: F. avena- ceum, culmorum, graminearum, langsethiae, poae, sporotrichioides, tricinctum during years studies. Yet, langsethiae not identified 2017, cerealis found years, 2016–2018. It should be noted that most frequent incidence for avenaceum tricinctum, which within range 25.8–44.0 %, (Zhdanova, 2002) it refers group frequently dispersed species. PCR diagnostics Kyiv region, where studies evaluate spelt- like (Bila Tserkva Breeding Research Station (BTBRS) Institute Bioenergy Crops Sugar Beet NAAS (Mala Vilshanka village, Bila district, region) demonstrated spreading six fungi: avenaceum, among high aggressiveness spikelets observed graminearum; medium – low tricinctum. On background obtained diversity disease agent, we evaluated blight, whose fluctuated 7.4–8.4 scores years. Under climatic favorable development, 2016 (from time spring vegetation restoration on March 01, full ripeness grain July 09, there 404.2 mm precipitation exceeded perennial indices 175 %) RM-1, RM-3, RM-4, RM-5, RM-6, RM-9, RM-10 variety, Lisova Pisnia, 7.0 scores. Conclusions. laboratory related geographic distribution give grounds assumption an expansive complex Forest- Steppe Ukraine: A close association between biology weather their environment used us isolate five (RM-9, RM-10, RM-4), had higher than average three 2016, pathogen RM-3 RM-4 reliably more resistant compared standard. RM-4) (2016–2018) introduced breeding programs initial obtain varieties, blight.